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. Author manuscript; available in PMC: 2012 Jun 13.
Published in final edited form as: J Biol Chem. 1997 Dec 12;272(50):31719–31724. doi: 10.1074/jbc.272.50.31719

Fig. 5. Analysis of DNA bending of H3.3 and H3.3 T → G arrest sites containing specifically located CPDs.

Fig. 5

A, autoradiogram of ligated multimers of damaged H3.3 and H3.3 T → G on 8% nondenaturing polyacrylamide gel. The actual length of ligated multimers in base pairs is shown on the right. Lanes C, ligated multimers of undamaged H3.3 and H3.3 T → G. PRE, photoreactivating enzyme. M, ϕX174 DNA/HaeIII molecular mass markers. B, quantitation of DNA bending. RL is the ratio of the apparent length of the DNA fragment determined by comparison of the ϕX174 DNA/HaeIII molecular mass marker with its actual length.