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Serial passages on coated Matrigel. Single cells derived from collagenase-isolated limbal clusters from one limbal segment of the same donor as in Figure 1 were serially passaged on coated Matrigel in ESCM with BFGF and LIF. They generated spindle cells from P1 to P12 (A) and had a steady proliferative rate with the doubling time of 43 to 47 hours from P2 to P10 (B). Bar = 100 μm.
