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. 2011 Oct;2(10):979–984. doi: 10.1177/1947601911436009

Figure 2.

Figure 2.

Co-expression of ALDH1A1 with NFYA and Oct-1 in endometrial adenocarcinoma and the preferential expression of alternatively spliced isoforms of NFYA. Double staining of ALDH1A1 with NFYA (A and B) and with Oct-1 (C and D). ALDH1A1 expression is shown in brown (A) and NFYA in red (B). Tumor cells expressing both ALDH1A1 and NFYA are shown with arrows and those expressing NFYA alone with arrowheads. ALDH1A1 expression is shown in brown (C) and Oct-1 in red (D). Tumor cells expressing both ALDH1A1 and Oct-1 are shown with arrows and those expressing Oct-1 alone with arrowheads. Magnification is 400×. (E) Dot blot analysis of ALDH-hi and ALDH-lo populations of HEC-1 and HEC-1A. These 2 populations are boxed. (F) RT-PCR analysis of NFYA-long and NFYA-short in the sorted ALDH-hi and ALDH-lo populations. The intensity of both NFYA-long and NFYA-short is quantified, and its ratio is shown. Experiments were repeated 3 times, and the representative result is shown.