Table 1.
SAXS results; Bragg peak qhkl positions (using Miller Indices), d-spacing and Bragg peak ratios, nanostructure, and unit cell spacing (a) for HIIc phases, for lipoplexes of reduced or oxidized BFDMA/pGFP (φDOPE = 0) and reduced or oxidized BFDMA-DOPE/pGFP (φDOPE=0.71) with and without the presence of OptiMEM and 50% BS (v/v), and lipids of reduced BFDMA, reduced BFDMA-DOPE and DOPE. Errors are based on equipment calibration of 2% using a silver behenate standard.
| Lipoplex | Bragg peak qhkl position (nm−1) | Bragg peak ratios | Nano- structure |
d or aa (nm) |
|||||
|---|---|---|---|---|---|---|---|---|---|
| 1st peak | 2nd peak | 3rd peak | 4th peak | 2nd/1st | 3rd/1st | 4th/1st | |||
| Lipoplexes in 1 mM Li2SO4 | |||||||||
| BFDMARED/pGFP | q001 = 1.27 | q002 = 2.53 | - | - | 1.99 | - | - | Lαc | 4.95 ± 0.10 |
| BFDMARED-DOPE/pGFP | q10 = 1.12 | q11 = 1.97 | - | - | 1.76 | - | - | HIIc | 6.48 ± 0.13 |
| BFDMAOX/pGFP | - | - | - | - | - | - | - | - | - |
| BFDMAOX DOPE/pGFP | q10 = 1.12 | q11 = 1.97 | - | - | 1.76 | - | - | HIIc | 6.48 ± 0.13 |
| DOPE/pGFP | q10 = 0.95 | q11 = 1.67 | q20 = 1.92 | - | 1.75 | 2.00 | - | HIIc | 7.63 ± 0.15 |
| Lipoplexes in OptiMEM and 50% v/v BS | |||||||||
| BFDMARED/pGFP | q001= 1.13 | q002 = 2.25 | - | - | 1.99 | - | - | Lαc | 5.56 ± 0.11 |
| BFDMARED-DOPE/pGFP | q10 = 1.04 | q11 = 1.79 | q20 = 2.08 | q21 = 2.77 | 1.72 | 2.00 | 2.66 | HIIc | 6.97 ± 0.14 |
| BFDMAOX/pGFP | - | - | - | - | - | - | - | - | - |
| BFDMAOX-DOPE/pGFP | q10 = 1.04 | - | - | - | - | - | - | - | 6.04 ± 0.12 |
| DOPE/pGFP | - | - | - | - | - | - | - | - | - |
| Lipids only in 1 mM Li2SO4 | |||||||||
| BFDMARED | - | - | - | - | - | - | - | - | - |
| BFDMARED-DOPE | q10 = 1.05 | q11 = 1.81 | q20 = 2.12 | - | 1.72 | 2.02 | - | HII | 6.91 ± 0.14 |
| DOPE | q10 = 0.95 | q11 = 1.69 | q20 = 1.91 | - | 1.78 | 2.01 | - | HII | 7.63 ± 0.15 |
a
d-spacing, d given for lamellar Lα structure, a=2/√3d given for hexagonal HII structure (See Fig. 1C and 1D).