TABLE 1.
MS platforms and proteomic studies of food components in cancer prevention research1
| Food component | Model | Proteomic platform | Combined effects of food components on biological processes | Reference |
| Cruciferous | ||||
| BITC, PEITC, SFN | Lung, A549 | 2D-nano RPLC-MALDI-TOF/TOF | Disruption of cytoskeleton organization | 48 |
| BITC, PEITC, SFN | Breast, Ras-MCF10A | LC-ESI-Q-TOF | Induction of apoptosis | 69 |
| SFN | Breast, MCF10, MCF12A | SILAC, LC-QTOF | Upregulation of hydroxysteroid metabolism | 59 |
| SFN | Prostate, LNCaP | 2D-MALDI-TOF/TOF | Induction of apoptosis | 47 |
| SFN | Colon, Caco-2 | 2D-MALDI-TOF | Decrease of neurotransmitter receptors | 46 |
| SFN | Liver, huh-7 | 2D-MALDI-TOF | Induction of apoptosis | 70 |
| PEITC | Liver, HepG2 | 2D-MALDI-TOF/TOF | Proapoptotic, antiinflammatory | 71 |
| PEITC | Plasma, TRAMP mice | 2D-MALDI-TOF/TOF | Induction of autophagy | 72 |
| PEITC | Cervix, Hela | ESI-LTQ | Inhibition of proinflammatory cytokine (MIF) | 73 |
| Indol-3-carbinol | Lung, A/J mice | iTRAQ-SCX-LC-TOF/TOF | Detoxification, antiproliferative | 60 |
| Brussels Sprouts | PBMC, human | 2D-LC-nanospray-MS | Growth arrest, proapoptotic, antioxidant | 74 |
| Brassica Sp. | Serum, human | MALDI-TOF | Inhibition of receptor tyrosine kinase | 75 |
| Brassica Sp. | Serum, human | MALDI-TOF | Reduced lipolysis in GSTM1+ subjects | 52 |
| Vegetable diets | ||||
| Mixture | Colon, C57BL6 mice | 2D-MALDI-TOF | Maintenance of intracolonic pH, | 76 |
| Antioxidant | Brain, canine | 2D-MALDI-TOF | Reduced protein oxidation | 77 |
| Isoflavones | ||||
| Genistein | Breast, Sprague-Dawley rat | 2D-MALDI-TOF | Increased differentiation | 78 |
| Genistein | Breast, Sprague Dawley rat | 2-D-MALDI-TOF/TOF,LC-ESI-MS/MS | Reduced EGFR signaling | 49 |
| Genistein | Stomach, SGC-7901 | SILAC-SCX-LC-trap/Orbitrap | Impaired signaling, cell growth, invasion | 37 |
| Genistein | Leukemia, HL-60 | 2D-MALDI-TOF/TOF | Proapoptotic | 79 |
| Genistein | Stomach, SGC-7901 | SILAC, SCX-LC-LTQ-Orbitrap | Induction of G2/M arrest and apoptosis | 53 |
| Genistein | Endothelial, EA.hy 926 | 2D-MALDI-TOF | Increased detoxification-GST | 80 |
| Soy isoflavones | PBMC, human | 2D-MALDI-TOF | Antiinflammatory | 38 |
| Red clover isoflavones | Liver, Sprague Dawley rat | 2D-MALDI-TOF | Reduced levels of 3a-hydroxysteroid-dehydrogenase | 81 |
| Flavonoids | ||||
| Quercetin | Colon, HT29 | 2D-MALDI-TOF | Proapoptotic, disruption of cytoskeleton | 82 |
| Quercetin | Colon, F344 rat | MALDI-FT-ICR MALDI-TOF/TOF | Reduced glycolysis, increased fatty acid oxidation, proapoptotic | 83 |
| Quercetin | Colon, SW480 | 2D-MALDI-TOF/TOF | Antiangiogenic | 84 |
| Quercetin | Liver, HepG2 | SILAC-NanoHPLC-ESI-Q-TOF | Disruption of cytoskeleton, antiangiogenic | 85 |
| Quercetin | Neuroblastoma, SJ-N-KP | HPLC-ESI-Q-TOF | Antiproliferative, proapoptotic | 86 |
| Quercetin | Ovary, 2774 | 2D-SELDI-TOF | Proapoptotic | 87 |
| Quercetin | Prostate, PC-3 | 2D-NanoHPLC-ion trap | Proapoptotic, reduced glycolysis | 88 |
| Flavone | Colon, HT-29 | 2D-MALDI-TOF | Antiproliferative, proapoptotic | 89 |
| Fatty acids and cyclopentenone PG | ||||
| Fish oil | Serum, human | 2D-MALDI-TOF, NanoLC-Q-Trap | Inhibition of acute-phase response, antiinflammatory | 90 |
| Fish oil | Liver, APOE3*Leiden Mice | 2D-MALDI-TOF | Inhibition of hypoxia, enhanced fatty acid oxidation | 91 |
| t10,c12-CLA | Liver, E3*Leiden, mice | 2D-MALDI-TOF | Increased lipid storage | 91 |
| Liver, APOE−/−, mice | 2D-LC-ESI-MALDI/TOF | Inhibition of TCA cycle, stimulation of gluconeogenesis | 92 | |
| c9,t11-CLA | Liver, APOE−/−, mice | 2D-LC-ESI-MALDI/TOF | Antiproliferative, antiinflammatory | 92 |
| Butyrate | Colon, HCT-116 | iTRAQ/ICAT-2D-LC-MALDI-MS | Antiproliferative, proapoptotic, inhibition of glycolysis, increased oxidative phosphorylation | 93 |
| Butyrate | Colon, HT-29 | 2D-MALDI-TOF | Proapoptotic | 94 |
| Butyrate | Colon, HT-29 | 2D-MALDI-TOF/TOF | Antiproliferative, proapoptotic, disruption of glycolytic pathways | 95 |
| PGA1 | Fibroblast, NIH-3T3 | NanoHPLC-ESI-Q/TOF | Disruption of cytoskeleton, proapoptotic | 96 |
| Grape compounds | ||||
| Grape seed extracts | Brain, Sprague Dawley rat | 2D-MALDI/-TOF 2D-RPLC-ESI-D-TOF | Neuroprotective | 97 |
| Resveratrol | Lymphoma, Jeko-1 | 2D-Nano RP-HPLC-ESI–ion trap, Q-TOF | Proapoptotic | 45 |
| Resveratrol | Colon, HCT-116(Bax+/−) | 2D-MALDI-TOF/TOF | Induced proapoptotic signaling | 98 |
| Resveratrol | Prostate, LNCaP | 2D-MALDI-MS | Reduced glycolysis | 99 |
| Garlic | ||||
| Diallyl-trisulfide | Stomach, BGC823 | 2D-MALDi/TOF, 2D-LC-ion trap | Stimulation of apoptosis | 100 |
| Diallyl-trisulfide | Bone, Saos-2 | 2D-MALDI-TOF | Impaired signaling, disruption of cytoskeleton, proapoptotic | 101 |
| Tea compounds | ||||
| Green tea extracts | Lung, A549 | 2D-HPLC-ESI-Q-TOF | Inhibition of cell motility | 102 |
| EGCG | Neuroblastoma, SH-SY5Y | 2D-RPLC-ion trap | Neuroprotective | 103 |
| Theobroma cacao | ||||
| Procyanidin | Breast, MDA-MB-231,-436,-468; SKBR3 | Antibody protein array | Antiproliferative, proapoptotic | 104 |
| Selenium | ||||
| Selenomethyl-selenocysteine | Plasma, Wistar rat | 2D-MALDI-TOF | Stimulation of acute-phase response | 105 |
| Vitamins | ||||
| Retinoic acid | Breast, MCF-7 | 2D-MALDI-TOF | Cell cycle arrest, antiproliferative, proapoptotic | 106 |
| Retinoic acid | Neuroblastoma, SH-SY5Y | 2D-MALDI-TOF/TOF, iTRAQ-LC-NanoESI- Q-TOF | Alteration of mRNA splicing and translation | 107 |
| L-Ascorbic acid | Leukemia, NB4 | 2D-MALDI-MS | Alteration of contractile system | 108 |
| Folate | Plasma, human | 2D-NanoLC-Q-Trap | Activation of immune function | 109 |
| Folate deficiency | Colon, NCM460 | 2D-NanoLC-Q-Trap | Increased DNA damage, metastasis reduced phase II enzymes | 110 |
1
BITC, benzyl isothiocyanate; EGCG, (-)-epigallocatechin gallate; ESI, electrospray ionization; FT-ICR, Fourier transform-ion cyclotron resonance; ICAT, isotope-coded affinity tag; iTRAQ, isobaric tag for relative and absolute quantification; LTQ, linear trap quadrupole; MALDI, matrix-assisted laser desorption/ionization; PEITC, phenetyl isothiocyanate; RPLC, reverse-phase-LC; SELDI, surface-enhanced laser desorption/ionization; SFN, sulforaphane; SILAC, stable isotope-based labeling; SXC, strong cation exchange; TOF, time-of-flight; 2D, 2-dimensional gel electrophoresis; Q, quadrupole.