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. Author manuscript; available in PMC: 2012 Jun 13.
Published in final edited form as: Evol Dev. 2004 Jan-Feb;6(1):41–49. doi: 10.1111/j.1525-142x.2004.04009.x

Table 2.

Table 2A. Primer sequences used for PCR amplification and sequencing
Primer Primer Sequence 5′-3′ PCR Product Size
12Sai AAACTAGGATTAGATACCCTATTA T ∼400 base pairs
12Sbi AAGAGCGACGGGCGATGTGT
16Sa CGCCTGTTTATCAAAAACAT ∼400 base pairs
16Sb CTCCGGTTTGAACTCAGATCA
COIF TAAACTTCAGGGTGACCAAAAAATCA ∼ 600 base pairs
COIR GGTCAACAAATCATAAAGATATTGG
28Sa GACCCGTCTTGAAACACGGA ∼ 600 base pairs
28Sb TCGGAAACCAGCTAC
Table 2B. PCR amplification profiles
Loci Number of Cycles Hotstart Denaturation Annealing Extension Final Extension
12S 25 94°C for 2 min 94°C for 15 sec 42°C for 30 sec 72°C for 30 sec 72°C for 7 min
16S 25 94°C for 2 min 94°C for 15 sec 42°C for 30 sec 72°C for 30 sec 72°C for 7 min
CO1 25 94°C for 2 min 94°C for 15 sec 36°C for 30 sec 72°C for 30 sec 72°C for 7 min
28S 25–30 94°C for 2 min 94°C for 15 sec 40–49°C for 30 sec 72°C for 30 sec 72°C for 7 min