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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Am J Transplant. 2011 Dec 7;12(3):640–648. doi: 10.1111/j.1600-6143.2011.03869.x

Figure 3. B cells do not impact tvDTH suppression via indirect pathway.

Figure 3

PBMC from two patients with high circulating naive B-cell counts, TOL patient 002002 and SI patient 002055 were assayed directly (PBMC, filled squares), after being depleted of CD19+ B cells (open triangles) or after depletion and add-back of B-cell fraction (gray circles). Cell fractions were injected into the footpad with PBS (negative control, data not shown) with recall antigen (TT/DT or EBV, left panel), recall antigen and self-peptide (p37TE or HA-1R, middle panel) or recall antigen and donor peptide (p37MA, or HA-1H, right panel). Swelling was measured before and 24 h after each injection. Net swelling was determined by subtracting the swelling response obtained from the injection of the same cell fractions in PBS.