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. 2012 Jun 15;23(12):2352–2361. doi: 10.1091/mbc.E11-12-1059

FIGURE 4:

FIGURE 4:

Number of the FAM21 repeat dictates its endosomal localization. (A) Schematic representation of YFP-fused FAM21 truncation mutants and degree of early endosomal (EE) localization. (B) HeLa cells were transfected with control vector (YFP only) or various shFAM21/YFP-FAM21 dual suppression/rescue vectors. YFP-FAM21 fusion proteins (green) were analyzed for localization with EEA1 (red) via immunofluorescence.