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. 2012 Jun 15;23(12):2352–2361. doi: 10.1091/mbc.E11-12-1059

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© 2012 Jia et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — All three elements of the FAM21 motif contribute to binding the CSC. (A) Schematic representation of FAM21 constructs used and degree of retromer binding determined by GST pull-down. (B) GST pull-down of retromer CSC using FAM21 R₂₁ and its mutants 1–5. FAM21 R_20-21 is used as a positive control and GST as a negative control. Shown is Coomassie blue–stained gel of input and bound samples from GST pull-down assays. (C) GST pull-down of retromer CSC using FAM21 R₂₁ and mutant 6. Shown is Coomassie blue–stained gel of input and bound samples from GST pull-down assays.