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. 2012 Jun 15;23(12):2362–2372. doi: 10.1091/mbc.E11-09-0790

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© 2012 Ballim et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — RA activates Tbx3 expression during mouse embryonic development. (A) Whole-mount in situ hybridization with Tbx3 probe of forelimb and hindlimb explants from E10.5 and E11.5 ICR mouse embryos cultured in serum-free medium with serum extender, with or without 200 nM all-trans and 9-cis RA for 72 h. (B) Left, whole-mount in situ hybridization with RARβ probe of handplates and footplates from E12.5 embryo forelimbs (FL) and hindlimbs (HL) cultured with or without RA. Right, whole-mount in situ hybridization with c-Ret probe of genital tracts from E11.5 embryos cultured with or without RA. Arrow indicates the Wolffian duct. (C) Left, whole-mount in situ hybridization of Raldh2 wild-type and mutant E10.5 embryos using antisense Tbx3 probe. Right, whole-mount in situ hybridisation of a wild-type E10.5 embryo with a sense Tbx3 probe. Note that this embryo was damaged in dissection, and therefore two halves are shown. Genotypes are as indicated. (D) Whole-mount in situ hybridization showing Tbx3 expression in handplates and footplates from E13.5 Raldh2^+/− and Raldh^−/− embryos. Right, handplates and footplates from a stage-matched E13.5 ICR embryo for comparison.