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Site-directed mutagenesis in five HA1 subunit regions (A, B, C, D, E) was performed following the addition of N-linked glycosylation sites. Nine N-X-S/T motifs were created in the five regions: (1) 83NNT, (2) 86NNT, (3) 94NFT, (4) 127NSS, (5) 138NRT, (6) 156NTT, (7) 161NRS, (8) 182NDT, (9) 252NAT. Triplet amino acids are underlined. Arrows point away from wild-type sequences to amino acid changes resulting in N-linked glycosylation sequences.
