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. 2012 Jun 13;7(6):e39163. doi: 10.1371/journal.pone.0039163

Figure 2. NS5B double band formation in TGV-treated NS5B overexpressing cells.

Figure 2

A) NS5B Western blot of lysates collected from TGV-treated Lunet cells expressing full-length NS5B via a BacMam system. Overexpressing cells were treated for 16 hours with various concentrations of TGV and compared to lysates from replicon cells treated for 24 hours with 50 nM TGV or DMSO as a control. B) Western analysis of TGV-treated cells overexpressing a 21 amino acid C-terminally truncated NS5B (Δ21). C) Immunoprecipitation and Western blots of overexpressed Δ21 NS5B encoding an N- or C-terminal His tag. NS5B Western blotting of anti-His immunoprecipitated material and His Western blotting of anti-NS5B immunoprecipitated material are shown.