Table 2. Specificity and dilution of monoclonal antibodies.
Specificity | mAb | Dilution | References |
Tn (GalNAcαThr/Ser) | 1E3 | 1∶5 | Clausen and Hakomori, unpublished |
Sialyl-Tn (NeuAcα2-6GalNAcαThr/Ser) | TKH2 | 1∶100 | [59] |
T (Galβ1-3 GalNAcαThr/Ser) | 3C9 a | 1∶25 | [60] |
Sialyl-Lewisx (NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc-R) | KM93 | 1∶40 | Calbiochem |
Sialyl-Lewis a (NeuAcα2-3Galβ1-3(Fucα1-4)GlcNAc-R) | Ca19.9 | 1∶500 | Santa Cruz |
Sialyl-T antigen was detected using the anti-T antibody after neuraminidase treatment as described in Material and Methods.