Table 3. Primer sequences and Real-time PCR conditions for the analysis of gene expression in mice gastric mucosa.
Gene | Primers Real-time PCR | Amplicon (bp) | PCR | |
St6galnac6 | for 5′- GCAACAAGACGCTGCCGTCC - 3′ | 107 | ||
rev 5′- GGTGCACTCAGCCCGCTCAA - 3′ | ||||
Galntl4 | for 5′- CCCTCATTGGCTGTTTCATT - 3′ | 106 | ||
rev 5′ - CTGATCCCAAGCTCCACATT - 3′ | 10 min, 95°C | |||
St3gal2 | for 5′- ATGGCTACCTTGCCCTACCT - 3′ | 203 | 45 times | 15 s, 95°C |
rev 5′- GTCCAGACGGGTGAGATGTT - 3′ | 60 s, 60°C | |||
C1galt1 | for 5′- TGCAGATTCCAGCCAACATAAAGATGA - 3′ | 130 | ||
rev 5′- AGCTTTGACATGTTTGGCCTTTTTCTC - 3′ | ||||
Hprt1 | for 5′- AGCTACTGTAATGATCAGTCAACG - 3′ | 198 | ||
rev 5′- AGAGGTCCTTTTCACCAGCA - 3′ |