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. 2012 Jun 13;7(6):e38353. doi: 10.1371/journal.pone.0038353

Table 3. Primer sequences and Real-time PCR conditions for the analysis of gene expression in mice gastric mucosa.

Gene Primers Real-time PCR Amplicon (bp) PCR
St6galnac6 for 5′- GCAACAAGACGCTGCCGTCC - 3′ 107
rev 5′- GGTGCACTCAGCCCGCTCAA - 3′
Galntl4 for 5′- CCCTCATTGGCTGTTTCATT - 3′ 106
rev 5′ - CTGATCCCAAGCTCCACATT - 3′ 10 min, 95°C
St3gal2 for 5′- ATGGCTACCTTGCCCTACCT - 3′ 203 45 times 15 s, 95°C
rev 5′- GTCCAGACGGGTGAGATGTT - 3′ 60 s, 60°C
C1galt1 for 5′- TGCAGATTCCAGCCAACATAAAGATGA - 3′ 130
rev 5′- AGCTTTGACATGTTTGGCCTTTTTCTC - 3′
Hprt1 for 5′- AGCTACTGTAATGATCAGTCAACG - 3′ 198
rev 5′- AGAGGTCCTTTTCACCAGCA - 3′