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. 2012 Jun 13;7(6):e38894. doi: 10.1371/journal.pone.0038894

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Splenocytes were stained with antibodies specific for CD21, CD23 and IgM, and subjected to FCM analysis. CD23⁻ (A) and CD23⁺ (B) populations were gated separately and analyzed according to CD21 and IgM expression. Cell populations were defined using the following markers: T1 cells, CD23⁻IgM^hiCD21⁻; T2 B cells, CD23⁺IgM^hiCD21⁺; MZP cells, CD23⁺IgM^hiCD21^hi; MZ B cells, CD23⁻IgM^hiCD21^hi; and FO B cells, CD23⁺IgM^loCD21^lo. The numbers denote the percentages of cells in the indicated squares. Data are representative of five independent experiments. (B) The absolute numbers of splenic T1, T2 and MZP B cells were calculated from the FCM data in (A) and the total number of erythrocyte-depleted splenocytes. Data represent the mean±S.D. from five independent experiments.