Table 2. Thermal stability of parental and mutant GOx stains incubated at 50 °C.
GOx Strain | T 70% (hrs)1 | T 1/2 (hrs)1 | Corr. Coeff. (R2)2 |
Biozyme | 10.4±0.5 | 20.1±0.9 | 0.990 |
Parent | 14.8±1.1 | 28.8±1.9 | 0.992 |
T132S | 19.8±1.3 | 38.5±2.4 | 0.986 |
T132S/T56V | 20.4±1.0 | 39.6±2.4 | 0.981 |
T132S/V42Y | 17.4±0.7 | 33.8±2.5 | 0.974 |
V42Y | 20.7±1.1 | 40.3±2.3 | 0.991 |
Error corresponds to the standard deviation of GOx kinetic rate measurements performed in triplicate.
Correlation coefficients obtained from an exponential least squares regression fit of GOx kinetic rates measured in triplicate vs. time.