Skip to main content
. 2012 Jun 13;7(6):e38102. doi: 10.1371/journal.pone.0038102

Figure 5. Effect of APC depletion on known mediators of chromatin compaction.

Figure 5

A. Dynamics of chromatin association of indicated components of condensin I and II complexes, Topoisomerase IIa, Kif4 and two linker histones in mock-and APC-depleted extract, as determined in the proteomic screen is represented in a heat map, color-coded as in fig. 4. B . Average values from all histones identified in the screen (H2B, H2A, H2A.ZI2, H1C, hist1H4I and B4) normalized to corresponding total signal in mock-depleted samples. C . Immunoblotting of the chromatin samples prepared as for the proteomic screen, with antibodies recognizing Xenopus TopoII (top panel) and Smc2 (bottom panel). Each lane contains the equivalent of 200 ng chromatin.