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. 2012 Jun 13;7(6):e38709. doi: 10.1371/journal.pone.0038709

Figure 2. Protein synthesis inhibition by DATIN.

Figure 2

A: Inhibition of protein synthesis in vivo. There was a decrease in DATIN production in recombinant E. coli over-expressing Rv0079 gene after 10 hr of incubation. E. coli expressing H. pylori isocitrate dehydrogenase (HP0023) was taken as a control. B: Coupled in vitro transcription/translation of circular DNA templates using E. coli S30 Extract System for Circular DNA. Reaction mixtures contained Milli-Q water (lane 1), DATIN (lane 2) and BSA (lane 3) (an unrelated control). Full-length luciferase migrated at 61 kDa. An apparent internal translation start resulted in a second major gene product of 48 kDa.