Figure 3. Growth (in broth) of recombinant E. coli expressing DATIN.

Broth cultures from E. coli BL21(pRSETA+Rv0079) and E. coli BL21(pRSETA) were inoculated into Luria Bertani broth to give an initial OD (600 nm) of 0.04 and the cultures were incubated for 15 or 24 hr at 37°C under IPTG induction. The growth curves of the strains were generated by measurement of the OD at 600 nm. The values represent the mean of three independent shaking cultures with the standard deviation.