Figure 4. Growth (in broth) of recombinant M. bovis BCG over-expressing DATIN.

Broth cultures from BCG (pMV261+Rv0079) and BCG (pMV261) were inoculated into Middlebrook 7H9 broth to give an initial OD (600 nm) of 0.02 and the cultures were incubated at 37°C as static cultures. The growth curves of the strains were generated by measurement of the OD at 600 nm. The values represent mean of the three independent ODs/static cultures with standard deviation.