Table 1. Analysis of apoptosis in thymus.

Groupsa	3 days p.i.b		5 days p.i.b
	Annexin V+PI-±SE (%)	Annexin V+PI+±SE (%)	Annexin V+PI-±SE (%)	Annexin V+PI+±SE (%)
VK627	19.59±1.83c	1.16±0.12	16.82±1.37 c	2.87±0.32
rVK627E	9.43±1.52	1.21±0.17	10.36±1.01	1.84±0.12
TsE627	10.21±1.45d	1.12±0.28	11.01±1.13 d	1.13±0.23
rTsE627K	20.58±3.03	1.31±0.34	19.19±1.21	2.11±0.07
Mock	11.88±1.84	0.96±0.07	12.11±1.45	0.87±0.06

^aMice (n = 6/group) were i.n. infected with 10⁴ PFU of V_K627, rV_K627E, Ts_E627, rTs_E627K.

^bMice were euthanized on 3 day p.i. and 5 day p.i., and three thymuses per group per time point were prepared for single cell suspension. Following staining with AnnexinV and PI, apoptotic cells were analyzed by flow cytometry. The early apoptotic cells were determined by annexin V⁺/PI⁻ and late apoptotic cells were determined by annexin V⁺/PI⁺. The data shown represents mean ± SD for three independent experiments.

^c p<0.05 compared to rV_K627E;

^d p<0.05 compared to rTs_E627K.