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. 2012 Jun 13;7(6):e38542. doi: 10.1371/journal.pone.0038542

Figure 5. Trypsin digestion of full-length Lsr2 co-purified with E. coli genomic DNA.

Figure 5

Trypsin (1∶500) initiates the compaction of co-purified DNA by Lsr2 over 1 h as visualised on a 1% agarose gel (SYBR® safe DNA stain). Subsequent digestion of the compacted DNA using DNase shows protection of the DNA by Lsr2. Equivalent samples were run on an SDS-PAGE gel to show the presence of Lsr2 (both monomer and dimer forms) in the sample after trypsin digestion. Components are labeled: C  =  condensation of DNA upon treatment with trypsin; P  =  protection of DNA; D  =  Lsr2 dimer; M  =  Lsr2 monomer).