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Fluorescence decay of OGB-1 (O6806; Molecular Probes, Eugene, OR) at various calcium concentrations (calibration kit C3008MP; Molecular Probes), as indicated. Two-photon excitation at (~100-fs pulses; laser: MaiTai, Spectra Physics, Mountain View, CA), attenuated beam power 9 mW; 20× objective (XLUMPlanFL; Olympus, Tokyo, Japan). Modified from Gersbach and others (2009).
