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. 2012 Jun 8;7(6):e38426. doi: 10.1371/journal.pone.0038426

Figure 4. rraA contains putative SOS box unaffected by treatment with MTC or exposure to UV light.

Figure 4

(A) Putative rraA SOS box. (Top) Sequence of rraA promoter region showing putative SOS box sequence in red. (Middle) Proposed [6] consensus SOS box. (Bottom) Observed SOS box sequences in rraA promoter region. (B) Determination of RraA protein in exponential phase of treated and control SY2 cells to cells after treatment with MTC (2 mg/ml) or exposure to UV light. Aliquots were assayed by Western blotting at various time points and samples were separated by Criterion 12% Bis-Tris gel containing XT-MOPS buffer and immunoblotted using antibodies against RraA. Antibodies to ribosomal protein S1 were used to detect the internal loading control.