Parameters of hepatic inflammation. (A) Liver sections were stained for infiltrated macrophages and neutrophils (Mac-1), neutrophils (NIMP) and T cells (CD3), respectively, and counted. (B–E) Representative pictures of Mac-1 staining (200×) after feeding on the chow (B+C) and HFC diet (D+E) in non-immunized (B+D) and immunized (C+E) Ldlr−/− mice, respectively. (F) Gene expression analysis for tumour necrosis factor (Tnf), interleukin 6 (Il6) and 1β(Il1β) and monocyte chemoattractant protein 1 (Mcp1). * Significantly different from non-immunized mice on chow diet. *, ** and *** indicate p < 0.05, 0.01 and 0.001 respectively.