Table 2.
Analysis of tocopherol levels in the serum and mammary gland of ACI rats
| Serum
| ||||||
|---|---|---|---|---|---|---|
| α-T (μM) | γ-T (μM) | δ-T (μM) | α-CEHC (μM) | γ-CEHC (μM) | δ-CEHC (μM) | |
| 2 weeks | ||||||
| E2 Control | 33.8 ± 1.9 | 0.5 ± 0.1 | 0.1 ± 0.0 | 7.5 ± 1.0 | 0.3 ± 0.1 | 1.0 ± 0.2 |
| E2 + 0.3% γ-TmT | 44.6 ± 4.8* | 3.2 ± 0.2‡ | 0.5 ± 0.1‡ | 26.8 ± 2.6‡ | 17.8 ± 2.1‡ | 39.8 ± 6.2‡ |
| E2 + 0.5% γ-TmT | 34.2 ± 2.1 | 3.7 ± 0.5‡ | 0.6 ± 0.1‡ | 22.0 ± 1.1‡ | 25.6 ± 2.1‡ | 54.4 ± 4.2‡ |
| 10 weeks | ||||||
| E2 Control | 33.5 ± 1.5 | 0.5 ± 0.1 | 0.1 ± 0.0 | 4.7 ± 0.0 | 0.2 ± 0.0 | 0.6 ± 0.0 |
| E2 + 0.3% γ-TmT | 36.2 ± 0.6 | 3.2 ± 0.1‡ | 0.7 ± 0.1‡ | 14.0 ± 0.0‡ | 18.3 ± 0.0‡ | 32.3 ± 0.0‡ |
| E2 + 0.5% γ-TmT | 39.1 ± 1.7 | 7.0 ± 0.6‡ | 1.8 ± 0.2‡ | 16.1 ± 1.2‡ | 21.6 ± 2.8‡ | 44.1 ± 2.1‡ |
|
| ||||||
|
Mammary Gland
| ||||||
| α-T (μM) | γ-T (μM) | δ-T (μM) | α-CEHC (μM) | γ-CEHC (μM) | δ-CEHC (μM) | |
|
| ||||||
| 2 weeks | ||||||
| E2 Control | 25.5 ± 0.8 | 2.1 ± 1.2 | 0.1 ± 0.0 | 0.9 ± 0.1 | 0.0 ± 0.0 | 0.3 ± 0.1 |
| E2 + 0.3% γ-TmT | 37.1 ± 6.4 | 8.9 ± 2.4† | 2.4 ± 0.6† | 4.0 ± 0.9‡ | 3.7 ± 0.9‡ | 6.1 ± 1.6‡ |
| E2 + 0.5% γ-TmT | 47.0 ± 12.5 | 13.4 ± 2.8‡ | 3.7 ± 0.9‡ | 3.5 ± 0.3‡ | 4.9 ± 0.4‡ | 7.8 ± 0.5‡ |
| 10 weeks | ||||||
| E2 Control | 26.9 ± 0.8 | 0.8 ± 0.1 | 0.2 ± 0.1 | 0.4 ± 0.0 | 0.1 ± 0.0 | 0.1 ± 0.0 |
| E2 + 0.3% γ-TmT | 25.4 ± 0.8 | 6.4 ± 0.5‡ | 2.8 ± 0.2‡ | 1.4 ± 0.1‡ | 2.8 ± 0.2‡ | 4.5 ± 0.2‡ |
| E2 + 0.5% γ-TmT | 25.7 ± 1.6 | 9.1 ± 0.7‡ | 4.3 ± 0.3‡ | 1.8 ± 0.1‡ | 3.2 ± 0.2‡ | 6.0 ± 0.2‡ |
Serum and mammary gland samples were collected at necropsy and analyzed for α-, γ-, δ-tocopherol (α-, γ-, δ-T) and their short chain metabolites, carboxyethyl hydroxychromans (α-,γ-, δ-CEHC). β-Tocopherol is not shown because β-tocopherol and its metabolites were below the limit for detection. The data is expressed as the mean ± S.E.M (n = 6). Statistical significance,
*
p<0.05,
†
p<0.01,
‡
p<0.001.
p-Values are compared to the E2 control of their respective time points.