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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: J Mol Neurosci. 2011 May 21;45(3):409–421. doi: 10.1007/s12031-011-9549-8

Table 2.

Characteristics of the antibodies used in immunogold TEM

Antigen Poly/monoclonal Source Clone Dilution (IHC-P) Dilution (IEM)
FUS Polyclonal Sigma-Aldrich Inc., USA - 1:500 25μg/ml IgG
α-Internexin Monoclonal Cambridge Bioscience Ltd., UK 2E3 1:400 5μg/ml IgG
Ubiquitin Polyclonal DakoCytomation, Denmark - 1:1,000 25μg/ml IgG
NEFH-phosphorylated Monoclonal Sternberger Monoclonals Inc., USA SMI 31 1:1,000 1:100
NEFH non-phosphorylated Monoclonal Sternberger Monoclonals Inc., USA SMI 32 1:1,000 1:100
Polyglutamine Monoclonal Chemicon International Inc., USA 1C2 1:1,000 1:1,000

Abbreviations: FUS, fused in sarcoma; NEFH, neurofilament heavy polypeptide; IHC-P, immunohistochemistry using paraffin-embedded tissue; IEM, immunoelectron microscopy