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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: J Immunol. 2009 Dec 15;183(12):7919–7930. doi: 10.4049/jimmunol.0803903

FIGURE 6.

FIGURE 6

YF-17D elicits polyfunctional, long-lived memory CD8+ T cells. A, Kinetics of cytokine-producing CD8+ T cells in individuals immunized with YF-17D. PBMC were isolated from vaccinees and responses to the NS4B 214–222 peptide were measured by ICC staining. Representative flow plots (gated on total CD8+ T cells) from one vaccinee are shown. B, Functional profile of the YF-17D-specific CD8+ T cell response. All possible combinations of four functions (degranulation by CD107a staining and secretion of IFN-γ, TNF-α, and Mip-1β) are shown on the x-axis. Bars indicate the percentage of the total response contributed by CD8+ T cells with the combination of responses on the x-axis. Responses are grouped according to the number of functions and the data summarized by the pie charts. Each slice of the pie represents the fraction of the total response that consists of CD8+ T cells positive for a given number of functions. Mean data from four YF-17D-immunized individuals are shown. C, Functional profile of the YF-17D-specific memory CD8+ T cells 2 years after vaccination. Plots gated on CD8+ T cells show the cytokines produced in response to the stimulation of PBMC with the NS4B 214–222 peptide. Data from one representative vaccinee are shown.