Figure 4.

Phosphorylation of PKA targets in the amygdala following training with a single trial or two trials. A, Rats were given a single training trial and killed 4 min (n = 6), 60 min (n = 13), or 24 h (n = 6) later or (B) given two trials separated by 60 min (n = 8) and killed 1 h later. “Box” controls (n = 8) were exposed to the training apparatus twice separated by 60 min but did not experience the light or shock and were killed 1 h later. Home cage rats (n = 13) were transported on the day of training and killed from their home cage. Sixty minutes after a single trial there was a significant increase in the phosphorylation of MAPK (C), GLUR1 at SER845 (D) and CREB at SER 133 (E). One hour after two trials spaced by 60 min there was a significant increase in phosphorylation of GLUR1 (D) and CREB (E) in the amygdala, but no change in MAPK (C). Representative images from the Western blots are shown below each graph (*p < 0.05 vs HC; **p < 0.05 vs 4 min; #p < 0.05 vs 24 HR; ##p < 0.05 vs BOX).