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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Pain. 2011 Jul 22;152(10):2323–2332. doi: 10.1016/j.pain.2011.06.025

Figure 6.

Figure 6

(a) Oral SCC cells show dose-dependent increase in EDNRB mRNA expression in response to transduction with Ad-EDNRB HSC-3 cells are transduced at 12.5, 25, 50, 100, and 200 MOI. RT-PCR performed on each group reveals that there is a dose-dependent increase in EDNRB mRNA expression with increasing MOI. Fold change is calculated relative to nontransduced HSC-3. (b) Ad-EDNRB transduced oral SCC cells express high levels of ETB receptor compared to control oral SCC cells.

Immunofluorescence images are quantified using Cell Profiler software. ETB receptor expression is quantified, and relative expression is calculated based on nontransduced HSC-3 expression. Cells tranduced with Ad-GFP do not express high levels of ETB receptor. However, cells transduced with Ad-EDRN express 9.6 times more ETB receptor than control, nontransduced cells.