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Lithium treatment did not change Akt kinase activity after stroke. Akt kinase activity at 1, 5 and 24 h after ischemia was measured. Akt activity was determined by the amount of GSK3 substrate phosphorylated by Akt as detected by Western blot. Representative relative optical densities of protein bands from the ischemic penumbra are shown in the bar graphs. Optical densities were normalized to those of sham samples, which were set as 100%. n=4–5 per group. *P<0.05 vs. sham.
