Figure 5.

Neuron expressing BDNF-pHluorin and loaded with the Ca²⁺ indicator fura-2 AM (image is of 488 nm-excited BDNF-pHluorin). This cell was sensitive to pH (as in Figure 4) and responded to electrical stimulation with Ca²⁺ transients (using 380 nm excitation, as in Figure 5). Consistent with activity-dependent BDNF release, dendritic BDNF-pHluorin puncta show decreases in intensity (~20% deltaF/F) as a result of BDNF-pHluorin discharge from vesicles following full fusion.