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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: Biochem J. 2010 Jul 1;429(1):127–136. doi: 10.1042/BJ20091461

Figure 10. Functional effects of replacement of Lys-134 of LDB1 with Arg.

Figure 10

HEK293 cells were transfected with a reporter gene containing 3 copies of an Lhx3 binding site upstream of a minimal promoter linked to the luciferase coding sequence and expression vectors for Lhx3, wild type LDB1, LDB1-K134R or empty vector as indicated as well as an expression vector for β-galactosidase as an internal standard for transfection efficiency. Cell extracts were prepared 20 h later and assayed for luciferase and β-galactosidase activity. Luciferase activity was corrected for β-galactosidase activity and the results are presented as means +/− SEM obtained from three separate transfections.