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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: Biochem J. 2010 Jul 1;429(1):127–136. doi: 10.1042/BJ20091461

Figure 3. Mutation of Lys-134 of LDB1 enhances LDB1 expression and also eliminates RNF12-induced formation of a slower migrating form of LDB1.

Figure 3

(A) HEK293 cells were transfected with vectors for wild type or mutant FLAG-tagged LDB1 as indicated. At 20 h after transfection, whole cell extracts were prepared and the expression of FLAG-tagged LDB1 was assessed by denaturing gel electrophoresis and immunoblotting with FLAG monoclonal antibody. A horseradish peroxidase labeled anti-mouse secondary antibody was used with a chemiluminescent detection reagent to visualize the immunoreactive proteins. (B) Longer exposure of the immunoblot in (A). Proteins with the appropriate migration for full-length, FLAG-tagged LDB1 (closed arrow) and a more slowly migrating form of the protein (open arrow) were detected. (C) The immunoblot from (A) and (B) was stripped and incubated with antibody to ERK1 as a loading control.