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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: Biochem J. 2010 Jul 1;429(1):127–136. doi: 10.1042/BJ20091461

Figure 8. Ubiquitin-aldehyde protects ubiquitin fused LDB1 from de-ubiquitination in vitro.

Figure 8

A wheat germ based in vitro coupled transcription/translation system was used to express epitope tagged ubiquitin fused LDB1. All reactions contained the same reagents and template with the exception of ubiquitin-aldehyde which was present at the indicated concentrations. Aliquots of the reactions were resolved by denaturing polyacrylamide gel electrophoresis, transferred to a membrane and then incubated with FLAG antiserum to detect epitope-tagged LDB1. A horseradish peroxidase labeled anti-rabbit secondary antibody was used with a chemiluminescent detection reagent to visualize the immunoreactive proteins. The migration of a protein the appropriate size for the tagged-ubiquitin-LDB1 fusion is indicated with an open arrow.