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. 2012 Jun 14;8(6):e1002519. doi: 10.1371/journal.pcbi.1002519

Figure 3. 3D image visualization and analysis for measuring single-cell gene expression of C. elegans.

Figure 3

(a) Tri-view display of a confocal image of C. elegans (L1 stage). Green: DAPI staining (pseudo-colored); red: myo3:GFP labeled muscle cells. (b) Tri-view display of the 3D watershed segmented nuclei of (a). The co-localized image objects are indicated by crosses (white). (c) A spreadsheet display of 3D measured gene expression of various cells. All sub-figures are produced using VANO [39], a 3D annotation tool.