Skip to main content
. 2012 Jun 14;7(6):e39248. doi: 10.1371/journal.pone.0039248

Figure 2. Expression in bacteria or yeast does not substantially alter the primary structural features of MaviP35 and AcP35.

Figure 2

FLAG-tagged MaviP35 and AcP35 were purified from bacteria or yeast. (A) Each sample was analyzed using Matrix Assisted Laser Desorption Ionisation-Mass Spectrometry. (B) Reduced and alkylated proteins were subjected to trypsin digestion, then the peptides were analyzed by LC-ESI-MS. The intensity of peaks of masses corresponding to the predicted peptide mass of unmodified or modified amino terminal tryptic peptides were measured. Based on the assumption that the peak intensities are proportional to the peptide amount, the relative amount of each peptide was calculated. The integrities of the peptides were confirmed by MS/MS analysis.