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. 2012 Jun 14;7(6):e39091. doi: 10.1371/journal.pone.0039091

Figure 1. WAS gene expression is restricted to hematopoietic cells and hemogenic progenitors.

Figure 1

(A) Analysis of WAS gene expression in different cell lines. mRNA was obtained from hematopoietic cell lines (AlloT and Raji), hESCs-derived myeloid CFUs (And-1 CFUs), undifferentiated hESCs (AND-1 and SHEF-2), a human fibroblastic cell line (293T) and human endothelial cells (HUVEC) and analyzed by RT-PCR for WAS expression (see M&M for details). (B) Time-course analysis of WAS gene expression during hematopoietic differentiation of AND-1 and H9 hESCs (see M&M and Figure S1 for details). mRNA was extracted at different days during differentiation as indicated and WAS expression analyzed by RT-PCR. (C) Analysis of WAS gene expression in hemogenic progenitors and hematopoietic cells. CD45−CD31+ (containing hemogenic progenitors) and CD45+CD31+ (containing hematopoietic cells) populations were sorter from AND-1 (left) and H9 (right) hESCs after 15 days of hematopoietic differentiation. WAS mRNA relative levels were determined in sorted cells by RT-PCR. ΔCt value for WAS expression was obtained using GAPDH as reference gene in all experiments. CFUs: Colony Forming Units.