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. 2012 Jun 14;7(6):e39017. doi: 10.1371/journal.pone.0039017

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(A) Normal phase one-dimensional thin-layer chromatography (1D TLC) of saponified and esterified lipids from M. tuberculosis (M. tb), S. rugosus and S. rotundus. From left to right: Lanes 1, 2, 3, and 7 were loaded with purified keto (1), methoxy (2), alpha (3), or mixed (7) MAMEs of M. tuberculosis. Lanes 4, 5, 6 were loaded with methylated saponificates containing both fatty acyl methyl esters and MAMEs derived from S. rotundus (4), S. rugosus (5), or M. tuberculosis (M. tb, 6). (B) Positive-ion mode Q-Tof ESI-MS analysis of total MAMEs derived from M. tuberculosis (top panels), with insets showing mass values that deduce to chemical formulas having either 3 (α) or 4 (keto, methoxy) oxygen atoms and compared with MAMEs from S. rotundus and S. rugosus (middle and bottom panels).