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. 2012 Jun 14;8(6):e1002760. doi: 10.1371/journal.pgen.1002760

Figure 4. Phosphorylation of eIF2α during Mitochondrial Stress Requires GCN-2.

Figure 4

(A) Immunoblot of phospho-eIF2α from wild-type, clk-1(qm30) and clk-1(qm30);gcn-2(ok871) animals fed vector or gsp-1(RNAi). The total eIF2α and anti-HDEL immunoblots serve as loading controls. Synchronized animals were raised from eggs and harvested at the L4 stage. (B) Immunoblot of phospho-eIF2α from wild-type, isp-1(qm150) and isp-1(qm150);gcn-2(ok871) animals fed vector or gsp-1(RNAi). The anti-HDEL immunoblot serves as a loading control. Synchronized animals were raised from eggs on the indicated RNAi plates and harvested at the L4 stage. (C) Immunoblot of phospho-eIF2α from wild-type, clk-1(qm30), clk-1(qm30);gcn-2(ok871) or clk-1(qm30);pek-1(zcdf2) worms. The anti-HDEL immunoblot serves as a loading control. Synchronized animals were raised from eggs on vector(RNAi) plates and harvested at the L4 stage.