Figure 1. Stimulation of murine macrophages by human and GAS DNA.

(A–C) Bone marrow-derived mice macrophages (BMDMs) were stimulated with GAS and human genomic DNA (both 5 µg/ml) and secretion of IFN1, IFN-α and TNF-α in the supernatants measured. (D) Dose-dependency of GAS DNA-mediated stimulation (12 hours) of IFN-α and TNF-α secretion. Data were pooled from 3 experiments done in triplicates and presented as mean ± SEM. * P<0.05 ** P<0.01.