Figure 4. Inhibition of [³H]-Prazosin binding to α_1A-adrenoceptor by MT7, MT1 and chimeric toxins.

Binding experiments were performed by incubating α_1A-AR membrane fractions of receptor with [³H]-Prazosin (1.5 nM) and varying concentrations of toxin at room temperature and overnight. The results are expressed as the ratio of the specific [³H]-Prazosin binding measured with (B) or without toxin (B_o). All experiments were performed at least three times in duplicate.