Fig. 5.

CB₁ receptor inhibition attenuated diabetes-induced adhesion molecule production in vivo. a Representative images of VCAM-1 in mice retinal sections showing an approximately twofold increase in diabetic (Diab) mice as compared with vehicle (Veh)-treated controls. VCAM-1 was mainly localised within retinal capillaries; n=5, ×200 magnification, scale bar 25 μm. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer. c Statistical analysis of above (a) findings, expressed as relative optical density (ROD). b Western blot analysis showing increase in adhesion molecule ICAM-1 levels in diabetic animals as compared with the controls (n=5–6). Treatment of diabetic animals with SR 141716A (SR1) blocked these effects, but did not alter control levels. d Statistical analysis of above findings (b) showing 1.9-fold increase in diabetic animals.*p<0.05 vs vehicle group; ^†p<0.05 vs diabetes