Table 2. In vitro susceptibility of mutant strains to four antifungal agents.

	MIC80(µg ml−1)
Antifungal compounds	Amphotericin B	Fluconazole	Flucytosine	Caspofungin
H99	0.5	8	16	32
ubp5Δ	0.25	2	2	8
ubp5Δ+UBP5	0.5	8	16	32
doa4Δ	0.5	4	16	16
ubp7Δ	0.5	8	16	32
ubp13Δ	0.5	4	16	16
ubp14Δ	0.5	8	16	32
otu1Δ	0.5	8	16	32
rpn11Δ	0.5	8	16	32
ATCC22019	0.5	2	0.125	0.5

The MICs of four antifungal agents against C. neoformans WT, DUB mutants and reconstituted strains were determined by the Clinical and Laboratory Standards Institute broth microdilution refercence method (NCCLS, 2002), and Candida parapsilosis strain (ATCC22019) was used as a quality control strain.