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. 2012 Jun 14;8(6):e1002760. doi: 10.1371/journal.ppat.1002760

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Malone et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) A model of yfiBNR interaction. YfiN is a membrane-localized DGC controlled by YfiR. YfiB, the outer-membrane bound Pal-like protein, activates YfiN by sequestering YfiR. B) Co-immunoprecipitation of YfiN with flag-tagged YfiN and YfiR. Immunoblot of boiled M2 resin samples with anti-YfiN antiserum shows YfiN (lower band) co-precipitating with YfiN-flag (upper band) or YfiR-flag. C) Membrane localization of YfiB and YfiR. Immunoblots of fractionated membrane samples with anti-YfiB (upper panel) and M2 antisera (lower 2 panels). The left two panels show membrane fractions for PA01 yfiR-M2, the right panel for ΔyfiBN yfiR-M2. IM1/2: inner membrane fractions, OM1/2: outer membrane fractions.