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. 2012 Jun 14;8(6):e1002760. doi: 10.1371/journal.ppat.1002760

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Malone et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Colony morphologies of yfiBNR/dsbA single and double mutants on LB Congo-red agar. Indication of the following abbreviations: ΔBNR – ΔyfiBNR, ΔB – ΔyfiBNR Tn7::yfiNR, ΔR – ΔyfiR, pR-flag – pMR-yfiR-flag, pdsbA – pME6032ara-dsbA, pME – pME6032ara. B) Immunoblots of PA01 and ΔdsbA with M2 and anti-YfiN antisera, showing levels of YfiR-flag and YfiN in whole cell lysates. C) The effect of increasing concentrations of DTT on attachment (bars), of the ΔBNR and ΔB mutants is shown relative to wild-type PA01. Curves represent relative optical density with standard errors.