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. Author manuscript; available in PMC: 2012 Jun 14.
Published in final edited form as: Methods Mol Biol. 2009;522:319–330. doi: 10.1007/978-1-59745-413-1_21

Figure 2.

Figure 2

SMG epithelial rudiments cultured with FGF7 or FGF10. Figure 2-A shows epithelial rudiments cultured for 48 h and the corresponding bud expansion or duct elongation promoted by FGF7 or FGF10, respectively. Figure 2-B illustrates how to quantitate morphological events during SMG epithelial morphogenesis. The upper figure shows how the width is measured (indicated by arrowheads) and the number (*) of end buds is counted; the bottom figure shows that the diameter (dotted circle) and the length of the ducts (arrow) can be measured.