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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Stem Cell Rev. 2013 Aug;9(4):531–536. doi: 10.1007/s12015-012-9357-8

Figure 2.

Figure 2

Analysis of neural precursor cells. A&B) FACS analysis of neural and pluripotent stem cell marker expression in SCU-i10-derived NPCs at p3 - A) IgM isotype control (white) and NCAM (black), B) IgM isotype control (white) and Tra-1–60 (black); C&D) FACS analysis of neural and pluripotent stem cell marker expression in H1-derived NPCs at p2 – C) IgG1 isotype control (white) and Nestin (black), D) IgG2b isotype control (white) and Oct3/4 (black). E) RT-PCR analysis of neural and pluripotent stem cell marker expression in HSF-6-derived NPCs at p0 from EBs of different sizes. Data were generated using a Sabiosciences PCR array and were analyzed and normalized using the company’s proprietary control gene panel and software. F) Phase image of SCU-i10 NPCs at p3, day1. Scale bar=100µm; G) Immunostaining of SCU-i10 NPCs p3, day 3 for neural precursors with anti-Nestin (green) and for neurons with anti-TuJ1 (red). Hoechst stain (blue). Scale bar=100µm; H) Immunostaining of differentiated SCU-i10 NPCs p2 for all neurons with anti-TuJ1 (green) and for dopaminergic neurons with anti-tyrosine hydroxylase (red). Hoechst stain (blue). Cells were plated in NPM with bFGF and EGF for 4 days, growth factors withdrawn for 3 days and further differentiated in Neurobasal medium containing B27, BDNF and GDNF for 4 days. Scale bar=100µm.