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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Biomech Model Mechanobiol. 2012 Feb 4;12(1):5–17. doi: 10.1007/s10237-012-0377-8

Fig. 1.

Fig. 1

Calcific nodule formation following combination treatments of 15% equibiaxial strain (red bars) and 1 ng/ml of TGF-β1 (blue bars) over 48 hrs. Control (A), strain only (B), and TGF-β1 only (C) all show unperturbed monolayers, as does strain followed by TGF-β1 (D). TGF-β1 followed by strain reveals large, mature calcific nodules (E), while simultaneous TGF-β1 and strain cause small, less mature nodules (F). All treatments stained with Alizarin red to identify calcification. This experiment was independently replicated three times with identical results. Scale bar = 250 μm.