Table I.
Sequences and IC50 Values for GST-SNAPI Proteins
| Name | C-terminal sequence | IC50 value (μM) | S.D.a |
|---|---|---|---|
| WT(141-206) | -DEANQ--RATKLMGSG | 14.3 (2)b | 1.6 |
| R198A | -DEANQ--AATKLMGSG | 14.0 | 0.4 |
| R198E | -DEANQ--EATKLMGSG | 42.4 | 1.4 |
| R198T | -DEANQ--TATKLMGSG | 18.2 | 0.6 |
| A195S | -DESNQ--RATKLMGSG | 12.9 | 0.2 |
| 2H | -DEHH---ATKLMGSG | 42 | 6 |
| 3H | -DEHHH--ATKLMGSG | 125 | 19 |
| 4H | -DEHHHH-ATKLMGSG | 269 | 43 |
| 5H | -DEHHHHHATKLMGSG | 77 | 3 |
| EH | -DEHEHEHRRGL-GSG | 206 | 20 |
| HHR | -DEHH---RATKLMGSG | 118 | 17 |
| QHHR | -DEQHH--RATKLMGSG | 36 | 1 |
| HHHHR | -DEHHHH-RATKLMGSG | 42 | 10 |
| HHQHHR | -DEHHQHHRATKLMGSG | 131 | 18 |
| HHRR | -DEHH---RRATKLMGSG | 92 | 8 |
| HHHRR | -DEHHH--RRATKLMGSG | 28.3 (2)b | 2.2 |
| QHHRR | -DEQHH--RRATKLMGSG | 748 | 193 |
| HHHHRR | -DEHHHH-RRATKLMGSG | 56 | 10 |
| HHQHHRR | -DEHHQHHRRATKLMGSG | 27.5 | 1.4 |
| 1G | -DEANQG----RATKLMGSG | 1.3 (2)b | 0.1 |
| 2G | -DEANQGG---RATKLMGSG | 4.8 (2)b | 0.4 |
| 3G | -DEANQGGG--RATKLMGSG | 1.7 (3)b | 0.1 |
| 4G | -DEANQGGGG-RATKLMGSG | 6.3 | 0.4 |
| 5G | -DEANQGGGGGRATKLMGSG | 7.0 | 0.6 |
a
Standard deviation for IC50 was calculated from the variance for y values in curve fitting.
b
Number in parenthesis indicates multiple series of SNAPI dilutions were used for curve fitting.